Ely Triwijayanti, Anggela Hajar Puspitasari, Triyono Adi Purnomo, Nindi Novia Erin, Syifa Fajrisani, Arif Yachya, Yosephine Sri WulanManuhara
Gynura procumbens (Lour.) Merr. is a medicinal plant from Family Asteraceae commonly found in tropical Asia countries such as Indonesia. G. procumbens contains bioactive compounds such as triterpenoids, polyphenols, saponins, steroids, chlorogenic acid, caffeic acid, vanillic acid, coumaric acid, para hydroxybenzoic acid, flavonoids, and essential oils. This study aimed to determine phenolic compounds, flavonoids, and saponins content in the leaves, stems, and roots of G. procumbens. These bioactive compounds were determined by extraction method with methanol solvent and analyzed using thinlayer chromatography, Image-J software, and spectrophotometer. The results of the flavonoid content using T L C method showed that the leaves organ has the highest flavonoids content compared to other organs. The results of the saponin test using T L C did not show any saponin spot on the leaves, stems, roots of G. procumbens based on a standard solution of saponins. The results of analysianalysis results using a spectrophotometer showed that the total of phenolic (438.85 G A E mg/L for the total of phenolic compound content) and from the results of Image-J, it was found that the highest flavonoid color intensity was obtained in the leaf organ of G. procumbens. were the highest compared to the stem and root organs. Based on the results of this study, it can be concluded that the leaves organ of G. procumbens contained a lot of phenolic and flavonoid compounds content, while the content of saponin compounds contained was are so small that they are not detected by the T L C method. © 2022 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Faculty of Mathematics and Science, Universitas PGRI Adi Buana, Surabaya, Indonesia
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